Yeast respiration and fermentation

Two hundred millilitres of yeast suspension in a sealed vessel, stirred throughout. An oxygen probe and a carbon dioxide probe come down through the stopper, and a console records both channels against one clock. You inject the glucose yourself through the septum and read the slopes off the screen.

Loading the laboratory

O₂ – mg/LCO₂ – mg/Lt – min

Each step has a button in the rail; the bench makes the gesture. You can also grab the bottle and carry it yourself. Click a name or an object to look closer, double-click the bench to step back. Slide the view with shift + scroll, the arrow keys, a right-button drag or two fingers.

Step 1 of 13

1
Before you start
Three checks: goggles, the probe membranes, and a vessel that is genuinely sealed.
2
Start the recording
3
Read the baseline
4
Predict
5
Inject the glucose
6
What you see
7
Place the cursors
8
Read the rate
9
Why a straight line?
10
Let the oxygen run out
11
There is no oxygen left
12
Two pathways, one sugar
13
That is the run