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BiologyGuided practice
Biology
Temperature and enzyme activity
Biology · Guided practice
UNDERSTAND AN EXPERIMENT
A student investigates how temperature affects catalase, an enzyme that speeds up a reaction producing oxygen. She measures the volume of gas produced in one minute. The pH, amounts of enzyme and reactant, and measurement time are the same for each trial. A larger volume in one minute means a faster reaction.
Results
| Temperature (°C) | 10 | 25 | 37 | 45 | 60 |
|---|---|---|---|---|---|
| Oxygen in one minute (mL) | 4 | 11 | 19 | 12 | 1 |
1
At which tested temperature does the enzyme work fastest? Use a value from the table to support your answer.
read the results
2
The gas volume falls from 19 mL at 37 °C to 1 mL at 60 °C. How could a change in the shape of the enzyme’s active site explain this fall?
explain
3
The table does not establish the exact optimum temperature. Suggest two new temperatures to test to narrow it down, and one variable to keep constant. Explain your choices.
design a follow-up
Temperature and enzyme activity